NEIL1 (Nei Endonuclease VIII Like Protein 1, NEI1, hFPG1, FPG1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Nei-like protein 1, DNA glycosylase/AP lyase Neil1)

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£517.00

SKU:: 364328
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ser43-Ala314 of human Neil1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Bacterial Fpg/Nei proteins contain 2 structural domains: an N-terminal 2-layered beta sandwich and a C-terminal 4-helix bundle, which includes a helix-2-turns-helix (H2TH) motif and a zinc finger. The deduced 390aa human NEIL1 protein has conserved residues in 6 highly conserved regions that span both structural domains of the bacterial Fpg/Nei proteins, but it lacks the characteristic zinc finger motif. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice