NAGLU (N-Acetyl Alpha-D-Glucosaminidase, NAG, UFHSD1, NAGLU, Sanfilippo Disease IIIB, N-Acetylglucosaminidase,Alpha)

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£626.00

SKU:: 364290
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Val485-Trp743 of human NAGLU, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Weitere Details:: Weber et al. (1996) reported the cloning of the NAGLU gene by enzyme protein purification, amino acid sequence determination, and database searches. A TFASTA search aligned the amino acid sequence to sequence in the 5-prime flanking region of the 17-beta-hydroxysteroid dehydrogenase-1 gene (HSD17B1), and this sequence was used to screen cDNA libraries. Northern blot analysis showed a single 2.7-kb mRNA transcript with high expression levels in liver, ovary, and peripheral blood leukocytes and measurable amounts of transcript in other tissues. The full-length cDNA sequence was cloned into an expression vector, and cultured CHO cells transfected with this vector showed a 17-fold increase in enzyme expression. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VSHPDA GAAWRLLLRS VYNCSGEACR GHNRSPLVRR PSLQMNTSIW YNRSDVFEAW RLLLTSAPSL ATSPAFRYDL LDLTRQAVQE LVSLYYEEAR SAYLSKELAS LLRAGGVLAY ELLPALDEVL ASDSRFLLGS WLEQARAAAV SEAEADFYEQ NSRYQLTLWG PEGNILDYAN KQLAGLVANY YTPRWRLFLE ALVDSVAQGI PFQQHQFDKN VFQLEQAFVL SKQRYPSQPR GDTVDLAKKI FLKYYPRWVA GSW Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice