364235
MTR (5-Methyltetrahydrofolate Homocysteine Methyltransferase, CblG, MS, MeSe, MetH Methionine Synthase, Vitamin-B12 dependent methionine synthase)
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£626.00
- SKU:
- 364235
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser923-Asp1265 of human MTR, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- The remethylation of homocysteine to form methionine is catalyzed by the cytoplasmic enzyme 5-methyltetrahydrofolate-homocysteine S-methyltransferase (EC 2.1.1.13), which is also called methionine synthase. This enzyme requires methylcobalamin (MeCbl), a derivative of cobalamin, or vitamin B12, for activity. The patient was a 6-month-old girl with mental retardation, megaloblastic anemia, and high folate activity in the serum and red cells. For this reason and also because rodent and human forms of the enzyme were electrophoretically distinguishable, these workers could use binding to identify the presence of human methyltransferase (MTR) in rodent-human somatic cell hybrids. By this approach, they assigned the methyltransferase gene to chromosome 1. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice