364197
MPG1 (Macrophage Expressed Protein 1, MPEG1)
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£495.00
- SKU:
- 364197
- Reinheit:
- Purified by Protein A and peptide affinity chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys41-Ala313 from mouse MPG1, expressed in E. coli
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
- Weitere Details:
- Mpeg1 is threonine rich and shares 27.3% sequence identity with mouse perforin (PRF1) over an 88aa region predicted to form 2 antiparallel amphipathic helices involved in insertion into the lipid bilayer. Northern blot analysis detected a 4.2-kb MPG1 transcript in human myelomonocytic cells, both in monocytes and peritoneal macrophages. Northern blot analysis of normal mouse tissues showed high Mpeg1 expression in macrophages and weak expression in bone marrow. Mpeg1 expression in mouse cells and cell lines was also restricted to mature macrophage and macrophage-like cells, with increased Mpeg1 expression detected as progenitor cells differentiated into macrophages. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Versandbedingungen:
- Blue Ice