363965
LIG1 (ATP Dependent DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1)
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£524.00
- SKU:
- 363965
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gly484-Ile663 of human LIG1 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- The major DNA ligase activity in proliferating mammalian cells is due to the high molecular weight enzyme designated DNA ligase I. Barnes et al. (1990) cloned cDNA for human DNA ligase I, both by functional complementation of a Saccharomyces cerevisiae cdc9 mutation and by hybridization with oligonucleotide probes corresponding to peptide sequences of the purified bovine enzyme. Hybridization between the cloned sequences and mRNA as well as genomic DNA indicated that the human enzyme is transcribed from a single-copy gene. Barnes et al. (1990) mapped the DNA ligase I gene to chromosome 19 by hybridization to DNAs from well-characterized rodent-human somatic cell hybrids retaining subsets of human chromosomes. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice