KDM4A (Lysine Specific Demethylase 4A JMJD2, JMJD2A, JHDM3A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A)

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£626.00

SKU:: 363821
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Tyr141-Lys310 of human KDM4A expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: MJD2A encodes a deduced 1064aa protein. Like JMJD2B and JMJD2C, JMJD2A contains a JmjN domain, a JmjC domain, a JD2H domain, 2 TUDOR domains, and a bipartite nuclear localization signal that overlaps the C-terminal part of the second TUDOR domain. JmjN is a small domain found in the Jumonji family of transcription factors. JmjC is a putative enzymatic domain. JD2H is a region containing 2 cys-his clusters with weak similarity to a PHD domain. This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- YEKHVDEWNI GRLRTILDLV EKESGITIEG VNTPYLYFGM WKTSFAWHTE DMDLYSINYL HFGEPKSWYS VPPEHGKRLE RLAKGFFPGS AQSCEAFLRH KMTLISPLML KKYGIPFDKV TQEAGEFMIT FPYGYHAGFN HGFNCAESTN FATRRWIEYG KQAVLCSCRK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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