IKBIP (Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein, IKIP, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Beta Interacting Protein)

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£495.00

SKU:: 363610
Anwendung:: WB
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Thr78-Arg301 of mouse IKBIP, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Weitere Details:: IKIP orthologs are found in other species, including mouse, rat, pig, cow, and Xenopus laevis. Mouse and rat Ikip1 and Ikip2 encode 373- and 345aa proteins, respectively, and the human IKIP1 protein shows 77% and 79% identity with mouse and rat Ikip1, respectively. Northern blot analysis detected expression of 3 IKIP1 transcripts of 1.3, 1.6 and 2.9 kb in lung, kidney, spleen, thymus, and skeletal muscle. Immunofluorescence analysis showed that both transfected and endogenous IKIP1 localizes to the endoplasmic reticulum, and deletion of the hydrophobic region results in uniform distribution of IKIP1 throughout the cell. Tunicamycin and endoglycosidase H treatment demonstrated that IKIP1 and IKIP2 are glycosylated, and targeted mutation of IKIP1 showed that glycosylation occurs at asparagine-154. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TES SEFQGLQSKI SLISSKYQEC EALVEQLKAF QIAAHLKLLQ EEIHEMKTWS NRITERQGTL NNTLTRLSED IIKVDQGTAS MAKDMGLKIT SVKTDVRRIS GLVTEVESLT DAVQALGNKV KKVETATVEN IGDLLSSSID RTSALRKTAS ENAKRIDSVA QRLAELQGDF DEHTDRFLSL ESDRAKVLKA VSFANDLKPK VSNLKKDFSR LEPLVDDLTL R Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice