HSD17b12 (17-Beta-Hydroxysteroid Dehydrogenase Type 12, KAR, SDR12C1, Estradiol 17-beta-dehydrogenase 12, 3-Ketoacyl-CoA Reductase, Short Chain Dehydrogenase/Reductase Family 12C,Member 1)

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£626.00

SKU:: 363511
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Asn312 of human HSD17b12, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: HSD17B12 uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation. The human HSD17B12 gene encodes a protein of 312aa containing a dilysine endoplasmic reticulum (ER)-retention motif at the C terminus and 4 predicted transmembrane domains. Mouse and human HSD17B12 share 82%aa identity. Northern blot analysis showed expression of a 2.9-kb HSD17B12 transcript in all human tissues tested, with highest levels seen in liver, muscle, and kidney. Analysis of liver HSD17B12 mRNA in transgenic mice with altered levels of sterol regulatory element-binding proteins (SREBPs) showed no change in HSD17B12 expression compared to wildtype mice, suggesting that HSD17B12 is not regulated by SREBPs. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice