HSD17b1 (17-Beta-Hydroxysteroid Dehydrogenase Type 1, EDH17B2, EDHB17, HSD17, SDR28C1, Estradiol 17-Beta-Dehydrogenase 1, Short Chain Dehydrogenase/Reductase Family 28CE,1, 20 alpha-hydroxysteroid dehydrogenase)

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£626.00

SKU:: 363509
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Thr4-Pro289 of human HSD17b1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: HSD17B mRNA expression was studied by RT-PCR in 42 pituitary tumors and 3 normal pituitaries, HSD17B activity was studied in 11 tumors, and HSD17B1 was immunolocalized in vitro in 6 tumors. HSD17B1 gene expression was detected in 34 of 42 (81%) adenomas in all tumor subtypes; HSD17B2 mRNA was detected in 18 of 42 (43%) adenomas but not in prolactinomas; HSD17B3 mRNA was detected in 12 of 42 (29%) adenomas but not in corticotropinomas; and HSD17B4 was expressed in 20 of 42 (48%) adenomas by all adenoma subtypes. All 4 HSD17B isoforms were variably expressed in human anterior pituitary adenomas, which also showed HSD17B enzyme activity, suggesting that HSD17B may play an important role in regulating the local cellular levels of estradiol. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice