363444
Hepc (Hepcidin, HAMP, HFE2B, PLTR, LEAP1, Hepcidin Antimicrobial Peptide, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor)
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£524.00
- SKU:
- 363444
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln29-Thr82 of bovine Hepc, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Hepcidin is a peptide hormone produced by the liver discovered in 2000, that appears to be the master regulator of iron homeostasis in humans and other mammals. In humans, HAMP is the gene that encodes for hepcidin. Hepcidin preprohormone, prohormone and hormone size are 84, 60 and 25aa respectively. 20 and 22aa forms of Hepcidin also exist in the urine. The N terminal region is required for function, and deletion of 5 N-terminal peptides results in a loss of function. Hepcidin has a beta-sheet rich structure with 4 disulfide bonds. Hepcidin directly inhibits ferroportin, a protein that transports iron out of cells that store it. This prevents enterocytes of the intestines from secreting iron into the hepatic portal system, reducing iron absorption. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice