363392
GTF2E1 (General Transcription Factor IIE, Polypeptide 1, TFIIE-A, FE, TFIIE, TF2E1, TF2-E1, Transcription Factor II E1, General transcription factor IIE 56kD subunit, Transcription initiation factor IIE subunit alpha)
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£524.00
- SKU:
- 363392
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys15-Gln306 of human GTF2E1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter. Transcription factor II E (TFIIE) is one of several general transcription factors that make up the RNA polymerase II preinitiation complex. Transcription Factor II E is encoded by the GTF2E1 and GTF2E2 genes. TFIIE is thought to be involved in DNA melting at the promoter: it contains a zinc ribbon motif that can bind single stranded DNA.Human TFIIE consists of 2 subunits of relative molecular masses 56,000 and 34,000. The structure of TFIIE appears to be a heterotetramer of 2 alpha and 2 beta subunits, both subunits being required for optimal reconstituted basal-level transcription. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice