ERLIN2 (Endoplasmic Reticulum Lipid Raft Associated Protein 2, Erlin-2, SPFH2, NET32, C8orf2, ER Lipid Raft Associated Protein 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2)

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£517.00

SKU:: 363003
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Pro47-Asn339 of human ERLIN2, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: SPFH2 associated rapidly with activated IP3 receptors , which are substrates for the ERAD pathway, in various mammalian cell lines. A proportion of SPFH2 associated with several components of the ERAD pathway, and RNA interference-mediated depletion of SPFH2 inhibited polyubiquitination and degradation of IP3R1 and turnover of model ERAD substrates. The deduced 339- and 152aa proteins share the first 141 N-terminal aa, then diverge. Both proteins have an N-glycosylation site and type-2 membrane topology, and the longer protein has a lysine- and glutamic acid-rich region. Northern blot analysis detected ubiquitous expression of 1.6- and 2.5-kb transcripts; a minor 4.4-kb transcript was also observed. Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice