DSTYK (Dual Serine/Threonine And Tyrosine Protein Kinase, RIPK5, Dusty PK, RIP5, SgK496, Receptor Interacting Protein Kinase 5, RIP-homologous kinase, Sugen kinase 496, Receptor-interacting serine/threonine-protein kinase 5)

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£577.00

SKU:: 362896
Anwendung:: IHC-Fr
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa650-904 of rat DSTYK, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: The deduced 929aa protein contains a C-terminal kinase domain, which shares homology with the kinase domain of RIPK family members. Northern blot analysis of human tissues detected weak expression in heart, brain, placenta, skeletal muscle, kidney, pancreas, and testis. DSTYK failed to activate NF-kappa-B (NFKB1) in a reporter gene assay. Overexpression of DSTYK induced DNA fragmentation and cell death in a dose-dependent manner with cell death occurring approximately 30 hours after transfection. Caspase inhibitory protein and morphologic studies showed that DSTYK induced both caspase-dependent and caspase-independent cell death, and both the N- and C-terminal DSTYK deletion mutants retained the ability to induce cell death. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice