362866
DNAJC12 (DnaJ/HSP40 Homolog Subfamily C, Member 12, JDP1, J Domain Protein 1)
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£626.00
- SKU:
- 362866
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Lys183 of human DNAJC12, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Highly conserved C terminus, but lack the G/F-rich and CRR domains. Hahn et al. (1999) identified a J domain-containing protein in mouse and Drosophila. By database searching for sequences homologous to these proteins, Lee et al. (2000) identified a human homolog, which they designated JDP1, that has 2 isoforms generated by alternative use of polyadenylation sites. The primary isoform is 1.2 kb long and encodes a 198aa protein; the other is 0.7 kb long and encodes a 107aa protein. The 198aa protein shares approximately 77% sequence identity with the mouse protein. Northern blot analysis detected a single transcript of 1.3 kb at high levels in brain, heart, and testis, and at reduced levels in kidney and stomach. In mouse and Drosophila, expression is highest in kidney and restricted to heads, respectively. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice