362841
DHODH (Dihydroorotate Dehydrogenase, DHOdehase, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial)
Größe
£626.00
- SKU:
- 362841
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg35-Asp392 of human DHODH, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Dihydroorotate dehydrogenase (EC 1.3.3.1) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis. Minet et al. (1992) isolated a truncated human cDNA encoding this enzyme from a HeLa cell cDNA library by functional complementation of a corresponding deletion mutant from the Saccharomyces cerevisiae. The gene in yeast is symbolized ura1. DHO dehydrogenase is a monofunctional protein which, in most eukyeastaryotic organisms, is located on the outer surface of the inner mitochondrial membrane. In, however, it has a cytosolic location. Missense mutations of the DHODH gene cause Miller syndrome, an autosomal recessive disorder also known as postaxial acrofacial dysostosis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice