362639
COMP (Cartilage Oligomeric Matrix Protein, MED, EDM1, EPD1, PSACH, THBS5, TSP-5, Pseudoachondroplasia,Epiphyseal Dysplasia 1, Multiple, Thrombospondin 5)
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£524.00
- SKU:
- 362639
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser123-His240 of human COMP expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Weitere Details:
- Cartilage oligomeric matrix protein (COMP) is a member of the thrombospondin family of extracellular matrix proteins. The protein consists of five 87kD subunits held together by interchain disulfide bonds, forming a 435kD pentameric protein. COMP is expressed in all types of cartilage, vitreous of the eye, tendon, and vascular smooth muscle cells. Immunohistological staining of articular cartilage has revealed a developmentally regulated localization of COMP to the chondrocyte territorial and interterritorial matrix. COMP binds in a zinc-dependent manner to collagen type I and type II and also to collagen type IX. The thrombospondin family currently includes five members: TSP-1, TSP-2, TSP-3, TSP-4, and COMP (also referred to as TSP-5). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffn): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Positive Control Included: 362639A: COMP, Positive Control Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice