CLEC4C (C-Type Lectin Domain Family 4, Member C, CD303, CLECSF11,CLECSF7, HECL, DLEC, BDCA2, Blood dendritic cell antigen 2, C-type lectin superfamily member 7, Dendritic lectin)

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£524.00

SKU:: 362594
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Glu5-Ile183 of human CLEC4C expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: CLEC4C is a dendritic cell marker. By searching for sequences similar to mouse Nkcl, Fernandes et al. (2000) identified an EST clone isolated from a testis cDNA library that contained the full-length coding sequence for CLECSF11, which they designated HECL. The deduced 183aa protein has a calculated molecular mass of 20 kD and contains a C-type carbohydrate recognition domain (CRD) and 3 putative N-glycosylation sites, but no transmembrane domain or signal peptide. HECL shares significant homology with members of the C-type lectin superfamily, and its CRD is mose similar to that of mouse Nkcl. The 4 cysteines responsible for the C-type CRD fold are perfectly conserved between HECL and mouse Nkcl. Immunolocalization of epitope-tagged HECL expressed in COS cells revealed a cytoplasmic localization. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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