362541
CFLAR (CASP8 And FADD Like Apoptosis Regulator, CASH, CASP8AP1, CLARP, Casper, FLAME1, FLIP, I-FLICE, MRIT, USURPIN, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, Caspase-eight-related protein, FADD-like antiapoptotic molecule 1)
Größe
£533.00
- SKU:
- 362541
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg43-Tyr281 of rat CFLAR, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Caspases are cysteine proteases that play a central role in apoptosis. Caspase-8 (FLICE) may be the first enzyme of the proteolytic cascade that is activated by the FAS ligand (FASL) and tumor necrosis factor (TNF). Caspase-8 is recruited to FAS and TNF receptor-1 (TNFR1) through interaction of its prodomain with the death effector domain (DED) of the receptor-associating protein FADD. The predicted 480aa CASPER protein contains 2 DED-like modules at its N terminus and a C-terminal caspase-like protease domain. However, CASPER is not a caspase since it lacks several conserved amino acids found in all identified caspases. Northern blot analysis detected several CASPER transcripts, with highest expression in human skeletal muscle, pancreas, and heart. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Versandbedingungen:
- Blue Ice