362328
BTLA (B And T-Lymphocyte Attenuator, CD272, BTLA1, B-and T-lymphocyte-associated protein)
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£537.00
- SKU:
- 362328
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys30-Tyr183 of rat BTLA, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 and CTLA4 , BTLA interacts with a B7 homolog, B7H4. However, unlike PD-1 and CTLA-4, BTLA displays T-Cell inhibition via interaction with tumor necrosis family (TNF) receptors, not just the B7 family of cell surface receptors. BTLA is a ligand for tumor necrosis factor (ligand) superfamily, member 14 (TNFSF14), also known as herpes virus entry mediator (HVEM). BTLA-HVEM complexes negatively regulate T-cell immune responses. Northern blot analysis revealed expression in lymph node and spleen, as well as in polarized Th1 but not Th2 cells. Flow cytometric analysis demonstrated surface expression of a transmembrane protein. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Positive Control Included: 362328A: BLTA, Positive Control Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice