362269
bCMO1 (Beta-Carotene-15,15'-Monooxygenase 1, BCO1, BCDO, BCDO1, BCMO, Beta-carotene dioxygenase 1, Beta-carotene oxygenase 1, Beta, beta-carotene 15,15'-monooxygenase)
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£626.00
- SKU:
- 362269
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa6-206 of human bCMO1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. Beta-carotene 15,15-prime-monooxygenase (EC 1.13.11.21) is a key enzyme in beta-carotene metabolism to vitamin A. Beta Carotene 15,15-Monooxygenase 1 a protein that shares 36.7% sequence identity with the bovine retinal pigment epithelium-specific gene Rpe65. They found that recombinant Drosophila Bcdo catalyzes centric cleavage of beta-carotene, resulting in the formation of 2 molecules of retinal. RT-PCR detected Bcdo expression exclusively in Drosophila head. Bcmo1 as critical molecular player for the regulation of PPARγ activity in adipocytes. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice