362257
BCAN (Breast Cancer Anti-Estrogen Resistance 1, CAS, CRKAS, P130Cas, CASS, Crk-Associated Substrate, Cas Scaffolding Protein Family Member 1)
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£626.00
- SKU:
- 362257
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa19-427 of human TCN2, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- BCAR1, or CAS, is an Src family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion. The predicted 870aa BCAR1 protein has a calculated mass of 93kD. BCAR1 contains an SH3 domain in its N-terminal part, multiple potential tyrosine phosphorylation sites in its central part, and a proline-rich stretch in its C-terminal part. BCAR1 shares 91% amino acid identity with rat and mouse p130Cas. SDS-PAGE and Western blot analyses demonstrated that BCAR1 encodes a 116kD protein; the mouse and rodent p130Cas proteins exhibit the same size. BCAR1 transcript in all tissues tested, with highest expression in testis and low expression in liver, thymus, and peripheral blood leukocytes. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-3ug/ml Immunohistochemistry (Paraffin): 5-30ug/ml Immunocytochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice