b4GALNT2 (Beta-1,4-N-Acetyl Galactosaminyl Transferase 2, SD, B4GALGT2, B4GALT, Cad, GALGT2, Sda, Sd(a) beta-1,4-GalNAc transferase, UDP-GalNAc:Neu5Aca2-3Galb-R b1,4-N-acetylgalactosaminyltransferase)

Größe

£499.00

SKU:: 362247
Reinheit:: Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa324-566 of human b4GALNT2, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin 300, 50% glycerol.
Weitere Details:: B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen. The longer transcript encodes a deduced 566aa protein with a calculated molecular mass of 63.3kD. It contains an N-terminal cytosolic domain, followed by a transmembrane domain and a long catalytic domain. The shorter transcript encodes a deduced 506aa protein with calculated molecular mass of 57.0kD. It has a different N-terminal cytosolic domain than the full-length form. Northern blot analysis detected B4GALNT2 transcripts of 8.8, 6.1, 4.7, 3.8, and 1.65 kb. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-3ug/ml Immunohistochemistry (Paraffin): 5-30ug/ml Immunocytochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice