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362110

ANGPTL1 (Angiopoietin Like Protein 1, ANG3, ARP1, ANGPT3, AngY, Angioarrestin, Angiopoietin-3, Angiopoietin-related protein 1)

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£505.00

SKU:
362110
Reinheit:
Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to Leu141-Asp308 of rat ANGPTL2, fused to N-terminal His-Tag expressed in E. coli.
Format:
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:
The predicted 493aa ANGPTL2 protein contains the coiled-coil and fibrinogen-like domains that are conserved in angiopoietins. ANGPTL2 also has a putative N-terminal secretory signal sequence and 2 potential glycosylation sites. Human ANGPTL2 shares 95% amino acid sequence identity with mouse Angptl2, 59% identity with human ANGPTL1, 35% identity with mouse Angpt3 and human ANGPT4, and 34% identity with human ANGPT1 and ANGPT2. Recombinant ANGPTL2 expressed in mammalian cells was secreted and glycosylated. By Western blot analysis, recombinant ANGPTL2 had a molecular mass of ~64kD. Northern blot analysis of human adult tissues detected abundant expression of a major 4.0- and a minor 2.6-kb ANGPTL2 transcript in heart, small intestine, spleen, and stomach. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice