362043
AGC (Aggrecan, ACAN, AGC1, AGCAN, CSPG1, CSPCP, CSPGCP, MSK16, SEDK, Cartilage-specific proteoglycan core protein, Large Aggregating Proteoglycan, Chondroitin Sulfate Proteoglycan 1)
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£523.00
- SKU:
- 362043
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Glu2066-Arg2316 of bovine AGC, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Aggrecan, or large aggregating proteoglycan, is a proteoglycan, or a protein modified with carbohydrates; the human form of the protein is 2316aa long and can be expressed in multiple isoforms due to alternative splicing. Along with Type-II collagen, aggrecan forms a major structural component of cartilage, particularly articular cartilage. Aggrecan consists of two globular structural domains at the N-terminal end and one globular domain at the C-terminal end, separated by a large domain heavily modified with glycosaminoglycans. The two main modifier moieties are themselves arranged into distinct regions, a chondroitin sulfate and a keratan sulfate region. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EARLE IESSSPVHSG EESQTADTAT SPTDASIPAS AGGTDDSEAT TTDQKLCEEG WTKFQGHCYR HFPDRATWVD AESQCRKQQS HLSSIVTPEE QEFVNNNAQD YQWIGLNDKT IEGDFRWSDG HSLQFENWRP NQPDNFFATG EDCVVMIWHE KGEWNDVPCN YQLPFTCKKG TVACGEPPVV EHARIFGQKK DRYEINALVR YQCTEGFIQG HVPTIRCQPS GHWEEPRITC TDPATYKRRL QKRSSR Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice