362020
Adenylate Cyclase 8, Brain (HBAC1, AC8, ATP pyrophosphate-lyase 8, Adenylate cyclase type VIII, Ca(2+)/calmodulin-activated adenylyl cyclase)
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£524.00
- SKU:
- 362020
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys234-Glu371 of human ADCY8, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase.Ca(2+) stimulation of ADCY8 occurs through loosely bound calmodulin. By mutation analysis of the N-terminal calmodulin-binding domain of mammalian Adcy8, Gu and Cooper (1999) determined that this domain makes a minor contribution to the Ca(2+) stimulation of Adcy8 activity, although it may be involved in calmodulin trapping. Mutagenesis and functional assays of the C-terminal calmodulin-binding site indicated that this site is largely responsible for the Ca(2+) stimulation of Adcy8. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice