SIRL-1 (Signal inhibitory Receptor on Leucocytes-1, V-set and Transmembrane Domain-containing Protein 1)

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£1,170.00

SKU:: 353438
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Purified SIRL-1-hIg fusion protein in PBS
Klon:: 1A5
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Weitere Details:: In order to prevent host tissue damage immune activation has to be tighly regulated. Neutrophils and other myeloid cells have a pivotal role in controlling infection. They form the primary response at site of infection as they regulate replication of pathogens by phagocytosis, secretion of antimicrobial peptides and proinflammatory cytokines to activate the immune system. Without regulation these mediators can cause tissue damage or worse. To balance the immune response immune cells can express inhibitory receptors. The family of immune inhibitory receptors is still expending. The relatively new receptor SIRL-1 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing membrane protein expressed on human phagocytes. SIRL-1 contains two ITIMs in the intracellular tail, which are crucial for function. SIRL-1 can recruit the SH2-domain containing tyrosine phosphatases, SHP-1 and SHP-2, which may mediate the inhibitory effect. ITIM-bearing receptors inhibit signals relayed by immunoreceptor tyrosine-based activating motifs (ITAMs). ITAMs are the principal mediators of signal transduction for various receptors, including FcRs and C-type lectins. The ligation of SIRL-1 dampens Fc receptor induced ROS production in phagocytes, thereby impairing the microbicidal activity of neutrophils without affecting phagocytosis. Infectious stimuli cause rapid down- regulation of SIRL-1 expression. This allows microbial killing and clearance of pathogens. Neutrophil extracellular traps (NETs) have been implicated in the pathogenesis of systemic lupus erythematosus (SLE). Using anti SIRL-1 antibodies both spontaneous anti-neutrophil antibody induced NET formation is suppressed. This suggests a regulatory role for SIRL-1 in NET formation and a therapeutic target for regulation of SLE. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Functional Studies. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunoprecipitation: 1:50 Western Blot: 1:50, non-reduced Functional Studies: showed inhibition of FcepsilonRI-mediated degranulation by RBL-2H3 cells transfected with SIRL-1. The percentage of inhibition of degranulation by SIRL-1 was calculated by (OD405 TNP stimulation – OD405 TNP with SIRL-1 crosslinking)/(OD405 TNP stimulation – OD405 spontaneous release) x 100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice