HMGB1 (Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, SBP-1, High Mobility Group Box-1)

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£876.00

SKU:: 353419
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Hamster
Immunogen:: Recombinant protein corresponding to rat HMGB1.
Klon:: 5H6
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Weitere Details:: HMGB1 (High Mobility Group Box-1 protein also known as amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMGB1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMGB1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMGB1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 10ug/ml detected HMGB1 in NIH/3T3 cells. Flow Cytometry: staining cells were permeabilized with buffer containing 0.5% saponin. The cells were fixed in 0.01% formaldehyde before staining. Hamster IgG isotype control is used as a negative control. Western Blot: detects a band of ~30kD using reducing conditions. For non-reducing conditions a band is expected at both 27kD and 30kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice