353252
EAAT1 (Excitatory Amino Acid Transporter 1, EA6EAA1, EAAT 1, GLAST, GLAST1, Glast, GLAST-1, SLC1A3)
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£620.00
- SKU:
- 353252
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa14-42, RMERFQQGVRKRTLLAKKKVQNITKEDVK, of human EAAT1 at N-terminal, different from the related mouse sequence by 3aa and identical to the related rat sequence.
- Format:
- Supplied as a lyophilized powder from 5% BSA, 0.9% sodium chloride, 0.2% Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
- Weitere Details:
- Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. It is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). And this gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Furthermore, this gene acts as a symport by cotransporting sodium. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml. Boiling paraffin sections in 10mM citrate buffer, pH 6.0, for 20min is required for staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice