353202-BIOTIN
PKM2 (PKM, OIP3, PK2, PK3, Pyruvate Kinase Isozymes M1/M2, Cytosolic Thyroid Hormone-binding Protein, Opa-interacting Protein 3, Pyruvate Kinase 2/3, Pyruvate Kinase Muscle Isozyme, Thyroid Hormone-binding Protein 1, Tumor M2-PK, p58, MGC3932, OIP3, PK3, PKM, TCB, THBP1) (Biotin)
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£749.00
- SKU:
- 353202-BIOTIN
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli-expressed recombinant protein corresponding to aa1-531 of human PKM2.
- Klon:
- 160701
- Format:
- Supplied as a liquid in PBS, pH 7.4. Labeled with Biotin.
- Weitere Details:
- PKM2 (pyruvate kinase M2) in association with phospho-tyrosine (pTyr) motifs enables cells to use predominantly aerobic glycolysis instead of oxidative phosphorylation. Also this change may benefit cancer cells by enabling them to redirect glycolytic metabolites away from oxidation and energy production and, instead, toward anabolic processes and biosynthesis. PKM2-mediated generation of the aerobic glycolytic phenotype, although wasteful in terms of glucose consumption and waste generation, could provide a variety of advantages for cancerous cells. In addition, nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry, Immunofluorescence: 1:250 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice