351919
SMARCD3, NT (BAF60C, SWI/SNF-Related Matrix- Associated Actin-Dependent Regulator of Chromatin Subfamily D Member 3)
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£626.00
- SKU:
- 351919
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to human SMARCD3/BAF60C at N-terminal.
- Klon:
- D6F1S
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, <0.02% sodium azide, 50% glycerol.
- Weitere Details:
- ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). SMARCD3/BAF60C is essential for the development of the skeletal and cardiac muscle (10, 11). Knockdown of SMARCD3/ BAF60C using RNAi in mouse embryos leads to defects in heart development as well as abnormalities in the development of the cardiac and skeletal muscle mimicking the cardiac defects observed in congenital heart disease (12). In addition, SMARCD3/ BAF60C is part of the lipoBAF complex that remodels the chromaitn structure to activate lipogenic genes, promoting lipogenesis in response to feeding and insulin (13). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice