PDGF Receptor Alpha, phosphorylated (Tyr762) (Platelet Derived Growth Factor Receptor alpha, CD140A, PDGFR2, Rhe-PDGFRA)

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£659.00

SKU:: 351882
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to Tyr762 of human PDGF Receptor. Species sequence homology: mouse and rat
Klon:: D9B1N
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Weitere Details:: Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFRa) and PDGF receptor B (PDGFRB). PDGFRa and PDGFRB share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and C-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRa homodimers bind all PDGF isoforms except those containing PDGFD. PDGFRB homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor a/B binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRa and PDGFRB can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCgamma, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr762 is located in the activation loop of the PDGFRa kinase domain. Phosphorylation of PDGFRa at this site was also reported by several other labs to be a docking site for CrkII and CrkL upon induction by growth factor treatment (7,8). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice