351872
MYST2 (Histone Acetyltransferase MYST2, HBO1, Lysine Acetyltransferase 7, KAT7)
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£626.00
- SKU:
- 351872
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein surrrounding Val222 of human MYST2.
- Klon:
- D4N3F
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- MYST2, also known as HBO1 and lysine acetyltransferase 7 (KAT7), is the catalytic subunit of the HBO1 acetyltransferase complex, which consists of MYST2, MYST/ ESA1-associated factor 6 (MEAF6), inhibitor of growth protein 4 (ING4) or inhibitor of growth protein 5 (ING5), and one of two families of scaffold proteins (JADE-1/2/3 or BRPF1/2/3) (1,2). The substrate specificity of the HBO1 complex is determined by the associated scaffold protein. HBO1 complexes containing JADE scaffold proteins acetylate histone H4 on lysines 5, 8 and 12, while complexes containing BRPF scaffold proteins acetylate histone H3 on lysines 14 and 23 (2). In addition the scaffold protein appears to regulate the function of the HBO1 complex. Complexes containing JADE scaffold proteins bind to origin recognition complex 1 (ORC1) and regulate licensing of DNA replication, while HBO1 complexes containing BRPF scaffold proteins regulate transcription (2-5). MYST2 is required for regulation of cell proliferation (1), adipogenesis (6), embryonic development (7) and survival of fetal liver erythroblasts (8). In addition, MYST2 is over- expressed in several human cancers, including cancers of the testis, ovary, breast, stomach, esophagus, and bladder (9). The MYST2 gene is amplified and protein is over-expressed in breast cancers, and over-expression of MYST2 increases anchorage-independent growth of several breast cancer cell lines (10). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:1000 Immunoprecipitation: 1:100 ChIP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice