Amyloid, beta, NT (Amyloid beta A4 Protein, ABPP, APPI, APP, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, Beta-Amyloid Protein 42, Beta-APP42, Beta-Amyloid Protein 40, Beta-APP40, P3(42), P3(40), Gamma-Secretase C-Terminal fragment 59, Amyloid intracellular Domain 59, AICD-59, AID(59), Gamma-CTF(59), Gamma-Secretase C-Terminal fragment 57, Amyloid intracellular Domain 57, AICD-57, AID(57), Gamma-CTF(57), Gamma-Secretase C-Terminal fragment 50, Amyloid intracellular Domain 50, AICD-50, AID(50), Gamma-CTF(50), APP, A4, AD, Amyloid-beta, Amyloid beta, Amyloidbeta, beta Amyloid, betaAmyloid, Abeta)

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£537.00

SKU:: 351760
Reinheit:: Supernatant
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to human B-amyloid at N-terminal.
Klon:: D3D2N
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Amyloid B (AB) precursor protein (APP) is a 100-140kD transmembrane glycoprotein that exists as several isoforms (1). The aa sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released AB fragments form the main components of amyloid plaques in Alzheimer’s disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (frozen): 1:200 Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice