Alpha-E-Catenin (CTNA1, Catenin alpha-1, Cadherin-associated Protein, Renal Carcinoma Antigen NY-REN-13, CTNNA1)

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£626.00

SKU:: 351758
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Ala885 of human alpha-E-Catenin.
Klon:: D9R5E
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium- dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through B and alpha-catenin. alphaE-catenin is ubiquitously expressed, a-N-catenin is expressed in neuronal tissue, and a-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and alpha-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). Research studies also suggest that, rather than acting as a static link between cadherins and actin, a-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). alpha-catenin also plays a role in regulating B-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. alpha-catenin binds to B-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). Applications: Suitable for use in Immunofluorescence, Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (Immunocytochemistry): 1:800 Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice