SAP14, Recombinant, Human, aa1-125, His-Tag (SF3B14, SAP14, Splicing factor 3B 14kD Subunit, CGI-110, HSPC175, Ht006, P14, SF3B14a)

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£416.00

SKU:: 351487
Molekulargewicht:: 16.7
Reinheit:: ≥95% (SDS-PAGE)
Lagerbedingungen:: -20°C/-70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 5mM DTT, 1mM EDTA, 30% glycerol.
Weitere Details:: SF3B14, also known as SAP14, is a 125aa nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex. Recombinant protein corresponding to aa1-125 from human SF3B14, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~16.7kD (145aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.