NME3, Recombinant, Human, aa22-169, His-Tag (Nucleoside diphosphate Kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3)

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£371.00

SKU:: 351395
Molekulargewicht:: 19.1
Reinheit:: ≥95% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 2mM DTT, 50% glycerol.
Weitere Details:: NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Source: Recombinant protein corresponding to aa22-169 from human NME3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~19.1kD (169aa) Specific Activity: >150units/mg, and is defined as the amount of enzyme that convert 1umole each of ATP and TDP to ADP and TTP/minute at pH 7.5 at 25°C in a couple system with PK/LDH. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice