350863
MerTK (Mer, Receptor Tyrosine Protein Kinase Mer, c-Eyk, c-mer proto-oncogene tyrosine kinase, C-mer, EC 2.7.10, EC 2.7.10.1, MER receptor tyrosine kinase, MER, Receptor tyrosine kinase MerTK, RP38Proto-oncogene c-Mer, STK kinase, Tyrosine-protein kinase Mer)
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£1,017.00
- SKU:
- 350863
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to human Mer expressed in S. frugiperda insect ovarian cell line Sf 21, Arg26-Ala499. Species sequence homology: mouse (~20%), human (rh) Axl and rhDtk (~1%)
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken cEyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Iglike motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions. Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells Western Blot: 0.1ug/ml Knockout Validated: Mer is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in Mer knockout HepG2 cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice