HMGB1 (High-Mobility Group box1, HMG1, HMG3, SBP-1, High-Mobility Group box1, HMGB1, High-Mobility Group box1, High-Mobility Group (nonhistone Chromosomal) Protein 1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Protein 1)

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£438.00

SKU:: 349981
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-215 from human HMGB1 expressed in High Five (Trichoplusia ni) insect cells.
Klon:: J2E1
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Weitere Details:: High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-2000 Immunohistochemistry: 1:100-300 Optimal dilutions to be determined by the researcher. Hybridoma: FO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.