337417
TNF
Größe
£510.00
- SKU:
- 337417
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- C term -peptide of human SFPQ
- Format:
- Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- TNF-alpha, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-alpha is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-alpha are biologically active. TNF-alpha is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. Cellular response to TNF-alpha is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-kappaB) promotes the survival of cells, while TNF-alpha-mediated activation of caspase-8 leads to programmed cell death. TNF-alpha plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The role of TNF-alpha in autoimmunity is underscored by blocking TNF-alpha action to treat rheumatoid arthritis and Crohn’s disease. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice