331116
PCMT1 (Protein-L-isoaspartate (D-aspartate) O-Methyltransferase, PIMT, L-isoaspartyl Protein Carboxyl, Methyltransferase, Protein L-isoaspartyl/D-aspartyl, Methyltransferase, Protein-beta-aspartate Methyltransferase)
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£800.00
- SKU:
- 331116
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Human PCMT1
- Format:
- Supplied as a liquid in PBS, pH 7.3, 0.09% sodium azide, 50% glycerol.
- Weitere Details:
- PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase), also known as PIMT, is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family and is highly expressed in brain. Functioning as a monomer, PCMT localizes to the cytoplasm and participates in the degradation and/or repair of damaged proteins. More specifically, PCMT1 recognizes isomerized Asp or Asn residues in peptides and proteins and catalyzes the conversion of abnormal L-isoaspartyl and D-aspartyl residues to methyl esters that may then spontaneously hydrolyze to re-form normal aspartyl residues. In congruence with this reaction, PCMT1 converts the methyl donor S-adenosylmethionine (AdoMet) to S-andenosylhomocysteine (AdoHcy). In mice lacking PCMT1, damaged proteins accumulate in a variety of tissues and the ratio between AdoMet and AdoHcy is increased in brain tissue. The phenotypic result is progressive epilepsy and death at an early age. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice