VEGF (Vascular Endothelial Growth Factor)

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£691.00

SKU:: 301786
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Chicken/Avian
Immunogen:: Recombinant protein corresponding to human VEGF.
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, ampicillin and streptomycin, 0.05% sodium azide. Reconstitute with 30ul ddH2O.
Weitere Details:: Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that stimulates vasculogenesis and angiogenesis. VEGF belongs to a family of closely related growth factors that share a conserved pattern of eight cysteine residues and bind to common VEGF receptors. The VEGF family members bind in an overlapping manner to three receptor tyrosine kinases, VEGFR1, VEGFR2 and VEGFR3. Specifically, VEGF mediates angiogenic response by the activation of VEGFR2. VEGF is produced at high levels in endothelial cells but its expression can be found in other cell types, including fibroblasts, inflammatory cells, and many tumor cells, often in response to increasing tumor hypoxia via the HIF-1a pathway. Overexpression of VEGF can contribute to diseases such as cancer and vascular disease in the retina; also, VEGF is a cause of susceptibility to microvascular complications of diabetes type 1 such as diabetic retinopathy, diabetic nephropathy and diabetic neuropathy. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:500-1:2000 in cells fixed with paraformaldehyde Western Blot: 1:1000-1:8000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice