299091
Alpha1 Acid Glycoprotein (alpha1-AGP)
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£761.00
- SKU:
- 299091
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography from culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse Alpha1 Acid Glycoprotein.
- Klon:
- 15H2
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- a1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40-46kD member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 and -3 in Mus musculus). Circulating AGP-1 and -2 are both 189aa in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200-400ug/ml. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2-10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice