TRACP5 (TRAP5, Tartrate-Resistant-Acid Phosphatase 5)

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£991.00

SKU:: 298109
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: recombinant TRACP
Klon:: 162
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Weitere Details:: Monoclonal antibody TRACP5 recognizes human tartrate-resistant-acid phosphatase 5 (TRACP5). TRACP is considered a marker for differentiated monocytes like macrophages, dendritic cells and osteoclasts. It is also considered to be a serum marker for bone disease. TRACP has two isotypes (5a &5b) with different characteristics. Isoform 5a is intact and bears a sialic acid and has a lower activity and pH optimum (pH5.0-5.2) than TRACP5b (pH5.8-6.0). TRACP5a circulates as a 35kD glycoprotein and 5b as a linked heterodimer of proteolytically cleaved16 & 23kD fragments. TRACP5a has unique epitopes associated with the intact loop peptide. Serum TRACP protein is predominantly isoform 5a, while most of the activity is due to isoform 5b. TRACP5a, but not 5b is secreted by activated macrophages and dendritic cells and is therefore considered a marker for systemic macrophage and chronic inflammation. TRACP 5a levels are correlated positively with inflammation-related markers CRP, ferritin, and triglycerides, which are also elevated in cardiovascular patients (CVD), but have no relationship to TRAP 5b activity or other bone metabolic markers. TRACP5a is a specific secreted marker of inflammatory macrophage. TRACP5b is a marker for osteoclast number and bone resorption. The clinical significance of TRACP5a is not completely clear yet. TRACP5a levels are elevated in end stage renal disease, 30% of rheumatoid arthritis patients and was positively correlated with body mass and fat indices (adipose tissue macrophages). Elevated serum TRACP5a may be an indicator of advanced or systemic inflammatory disease associated with inflammatory vulnerable plaque and a risk marker for adverse events in CVD. Antibody TRACP5 recognizes the native conformation of TRACP5a and 5b. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:50 Western Blot: 1:50. The band size(s) are 14 and 35kD. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Granular cell tumor of the skin and ductal carcinoma of the breast Negative Control: Mouse IgG at 1ug/ml (Western Blot) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice