Toll-like Receptor 4 (TLR4, CD284, ARMD10)

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£876.00

SKU:: 298107
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant Toll-like Receptor 4
Klon:: 4D9
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Weitere Details:: The monoclonal antibody TLR4 reacts with the Toll-like receptor 4 (TLR4, CD284). TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single alpha-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double- stranded RNA from viruses.Toll-like receptor 4 has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. The extracellular TLR4 domain alone cannot bind to LPS, only the TLR4-MD-2 complex is capable of binding LPS. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro-inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferon-beta) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody TLR4 reacts with the extracellular part of the receptor. A few studies report the existing of soluble TLR4. This antibody should be able to recognize this protein. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:50 Western Blot: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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