Fibroblasts

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£876.00

SKU:: 298067
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Mouse thymic stromal cells
Klon:: ER-TR7
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Weitere Details:: Fibroblasts are the least specialized cells in the connective-tissue family. They are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix (ECM) that is rich in type I and/or type III collagen. Conective tissue consists of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. The activation of fibroblasts by inflammatory stimuli results in their migration, proliferation and deposition of extracellular matrix components, important features involved in both wound healing and fibrosis. The Fibroblasts antigen is a ubiquitous component of stromal (interstitial) matrix cartilage and of some basement membrane zones. The antigen detected is not a basement membrane component, nor any major collagen type or fibronectin. The antigen detected has a wider tissue distribution than reticulin. Fibroblasts detects an intracellular component of fibroblasts. Since Fibroblasts does not react with purified laminin, collagen types I-V, fibronectin, heparin sulfate proteoglycan, entactin or nidogen, it detects a hitherto uncharacterized antigen. The monoclonal antibody Fibroblasts can be used to study the micro-anatomy of various organs. Fibroblasts outlines the various compartments of peripheral lymphoid organs by characteristic labeling patterns (no such compartments are found in central lymphoid organs). Furthermore Fibroblasts delineates various types of connective tissue compartments in nonlymphoid organs. The antibody Fibroblasts detects reticular fibroblasts, which constitute the cellular framework of lymphoid and nonlymphoid organs and their products. Fibroblasts is useful to clearly delineated the follicles, periarteriolar lymphoid sheath and marginal zone; the major white pulp compartments. Furthermore in lymph nodes, the capsule, sinuses, follicles, paracortex and medullary cords are clearly delineated. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen/paraffin Optimal dilutions to be determined by the researcher. Positive Control: Spleen Negative Control: Lymphoid cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice