CD55 (DAF, Decay Accelerating Factor)

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£991.00

SKU:: 298053
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: NIH-3T3 cells expressing GPI-anchored rat DAF
Klon:: RDII-24
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Weitere Details:: The monoclonal antibody CD55 recognizes complement decay accelerating factor (DAF), also designated as CD55. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60kD transmembrane protein that binds C3b and C4b to inhibit formation and half- life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:50. A band size of 60-70kD. Immunohistochemistry(Frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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