Caveolin-1 (CAV1)

Größe

£1,138.00

SKU:: 298044
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes
Klon:: 7C8
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Weitere Details:: Caveolae are sphingomyelin/cholesterol-rich membrane domains first discovered as membrane invaginations on the surface of endothelial and epithelial cells. Caveolae are present in most cells, but are especially abundant in adipocytes. In addition to caveolins only two major protein components of caveolae were identified, namely the semicarbazide sensitive amine oxidase (SSAO) and the scavenger receptor CD36. Caveolin cycles between the plasma membrane and intracellular compartments via the endocytotic pathway. Caveolin is involved in the rapid intracellular transport of newly synthesized cholesterol from the ER directly to the caveolae. Caveolin plays an important role in multiple signaling pathways, molecular transport and cellular proliferation and differentiation. Caveolin binds to endothelial nitric oxide synthase leading to enzyme inhibition. Furthermore caveolin is a candidate tumor suppressor gene in many tumors. The specific functions of caveolin-1/caveolae are highly cell and context dependent. The monoclonal antibody Caveolin-1 recognizes caveolin-1alpha as well as caveolin-1beta, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat. The monoclonal antibody Caveolin-1 can be used to immuno-isolate caveolae. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:50. The band sizes are ~22kD (Caveolin-1beta) and ~25kD (caveolin-1alpha). Optimal dilutions to be determined by the researcher. Positive Control: Adipocytes (3T3-L1 adipocytes) Negative Control: Cytoplasmic extracts of adipocytes Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice