Basophils

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£876.00

SKU:: 298039
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Lysate of purified basophils
Klon:: 2D7
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Weitere Details:: The monoclonal antibody Basophils is a specific marker for basophils detecting a basophil-specific protein of 7.2-7.5kD localized in secretory granules. Activated basophils have been shown to have a reduced staining with the Basophils antibody consistent with localization of the antigen to secretory granules. Basophils are multifunctional haematopoietic cells that secrete a wide variety of proinflammatory agents upon activation such as histamine and cytokines. Moreover, basophils can induce IgE production by B cells. Under normal conditions basophils are primarily produced in the bone marrow, whereas mature basophils reside in the circulation and can enter tissues at sites of inflammation. Both basophils and mast cells have been implicated in the pathogenesis of allergic inflammation due to the abundant expression of high affinity receptors for IgE. The Basophils antibody can be used as a sensitive and precise marker for human basophils and does not react with lymphocytes, monocytes, eosinophils, neutrophils or mast cells in immunohistochemistry. Moreover, the antibody is suitable for western blot analysis of the Basophils antigen. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:50. The band sizes are 72 en 76kD. Immunohistochemistry(Frozen): 1:50 Immunohistochemistry(Paraffin): 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Chronic myeloid leukaemia (basophilia) Negative Control: CD203c negative cells (basophil depleted cell fractions) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice