254279
Ricinus communis (Agglutinin A chain, Agglutinin B chain, Castor bean, RCA 60, RCA60, Ricin A chain) (Not for Export EU)
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£728.00
- SKU:
- 254279
- Reinheit:
- Serum
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Purified castor bean lectin
- Format:
- Supplied as a liquid in PBS, 10mM sodium azide.
- Weitere Details:
- Ricinus communis agglutinin 60(RCA60) is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28,000 daltons and 32,000 daltons, termed A and B chain, respectively. The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell. The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell. Alone, the A chain is incapable of entering the cell and is thus not toxic. The A chain can be separated from the B chain and then linked by a cleavable disulfide bond to other proteins, such as antibodies. These antibody-toxin hybrid molecules can be used to kill specific cells with the appropriate antigenic determinant exposed, such as tumor markers on surfaces of malignant cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice