PP2A alpha, methylated (L309) (PP2A alpha, PP2Ac, PP2CA, PPP2CA, Protein Phosphatase 2, Catalytic Subunit, alpha isoform, Replication Protein C) (HRP)

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£774.00

SKU:: 254246
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe. The c-terminal sequence (as well as the carboxy-methylation) is absolutely conserved from human, mouse to yeast. Species sequence homology: Saccharomyces cerevisiae.
Klon:: 2A10
Format:: Supplied as a liquid in PBS, 0.01% thimerosal. Labeled with Horseradish peroxidase (HRP).
Weitere Details:: Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice